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Fig. S4
Inhibition of Stat3 in cardiomyocytes has no detectable effects during rapid animal growth. (A) Induction of dnStat3-GFP fluorescence in cardiomyocytes after 4-HT incubation in cmlc2:CreER;β-act2:RSdS or control β-act2:RSdS fish, during slow (normal) and accelerated growth conditions. (B) Control (β-act2:RSdS) and cmlc2:CreER;β-act2:RSdS animals each approximately doubled their mass after 10 d of rapid growth conditions. Data are mean ± SEM n = 12. (C) Representative images of cardiomyocyte proliferation using antibodies against PCNA and Mef2, indicating no detectable difference between control (β-act2:RSdS) and dnStat3-expressing fish (cmlc2:CreER; β-act2:RSdS) after 10 d of either slow or rapid growth. Quantified data are in Fig. 3D. (D) Control (β-act2:RSdS) and cmlc2:CreER;β-act2:RSdS embryos were incubated in 4-HT at 4 d postfertilization and allowed to grow to adulthood. Approximately half of the surviving animals from a clutch of 43 fish were cmlc2:CreER; β-act2:RSdS, indicated by myocardial dnStat3-GFP fluorescence. (Scale bars, 50 μm.)