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Fig. S7
prdm1aDBD-VP16 activating and prdm1aDBD-EnR repressing constructs are functional. (A-C) Injection of prdm1aDBD-EnR construct into prdm1a–/– embryos rescues prox1 expression in slow-twitch muscle (10% rescued in C, n=4/20) as shown previously (von Hofsten et al., 2008). (D-F) Injection of prdm1aDBD-VP16 construct into WT embryos shows precocious F310 immunoreactivity (arrows in F), similar to what is observed in prdm1a–/– embryos (57% exhibit F310 immunoreactivity shown in F, n=47/82). (G-I) Injection of prdm1aDBD alone into prdm1a–/– embryos does not rescue crestin expression in prdm1a mutant embryos (100% of prdm1a mutants injected with prdm1aDBD alone exhibit reduced neural crest as in I, n=10/10). (J-L) crestin trunk expression in 24-hpf WT/het embryos (J), prdm1a–/– embryos (K) and prdm1a–/– embryos rescued with full- length prdm1a mRNA (L).