ZFIN Image: Akitake et al., 2011, Fig. S1

Image

Figure Caption

Fig. S1 Dual reporter system to monitor Gal4-VP16 expression and UAS-driven transcription simultaneously. (A) Schematic diagram of the bicistronic construct used to generate transgenic lines for testing UAS copy number variants. Under the control of the EF1α promoter, Gal4- VP16 and mCherry are produced in equimolar amounts due to incorporation of the viral 2A peptide sequence. UAS copy number variants and the E1b minimal promoter were inserted into a multiple cloning site (MCS) upstream of GFP. (B) Transgenic F1 larvae generated with different UAS copy number variants show widespread, mCherry (left) and GFP (right) fluorescence at 2 dpf, but develop defects and do not survive to adulthood.

Acknowledgments

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Reprinted from Developmental Biology, 352(2), Akitake, C.M., Macurak, M., Halpern, M.E., and Goll, M.G., Transgenerational analysis of transcriptional silencing in zebrafish, 191-201, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.