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Fig. 9 Lack of pre-formed AChR clusters. Clusters of AChR in the muscle were stained with fluorescently labeled αBTX (red, post-synaptic AChRs) and projecting motor neurons stained with SV2 (green, pre-synaptic vesicles) antibody. At 28 hpf, pre-formed clusters containing AChR (arrows) were found dorsal to the migrating motor neuron tip in controls (A) while these were absent in appbMO injected embryos (B). Scale bar: 20 μm.
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Reprinted from Developmental Biology, 381(2), Abramsson, A., Kettunen, P., Banote, R.K., Lott, E., Li, M., Arner, A., and Zetterberg, H., The zebrafish amyloid precursor protein-b is required for motor neuron guidance and synapse formation, 377-88, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.