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Fig. S5
Dose-dependent rescue of miR-153 over-expression. (A) Single cell embryos were injected with a constant level of miR-153 and increasing amounts (increments of 50 pg) of snap-25a, snap-25b, or snap-25a&b mRNA. Embryo lysates from ~60 embryos were prepared from embryos in each treatment group and SNAP-25 protein levels were determined by western blotting. (B) Quantitation of westerns (n = 3) from A. The grey circles represent the amounts used in co-injection rescue experiments (75 pg each of snap-25a and b, 250 pg of snap-25a, and 300 pg of snap-25b).
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