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Fig. 4 Morphological defects exhibited in ztfpi-2 morphants. (A) SP-MO injected and p53-MO co-injected embryos displayed malformation of the MHB with pigment deficiencies 25 hpf but embryos exposed to Mcon-MO and Scon-MO did not (arrow). These morphological phenotypes were found to be rescued by concomitant injection of ztfpi-2 mRNA. (B) ztfpi-2-deficient embryos were partially rescued by co-injection of 75 pg ztfpi-2, 155 pg her4, and 85 pg mib mRNA. A total of 100 embryos were used per group. Representative results of three independent experiments are shown.
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Reprinted from Developmental Biology, 381(1), Zhang, Y., Wang, L., Zhou, W., Wang, H., Zhang, J., Deng, S., Li, W., Li, H., Mao, Z., and Ma, D., Tissue factor pathway inhibitor-2: A novel gene involved in zebrafish central nervous system development, 38-49, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.