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Fig. 3
Dorsomorphin promotes the growth of spermatogonia in 3 week cultures.
(A) RT-PCR results showing that feeder cells (OFC3LF+OFC3LG) express bmp2a, bmp2b and bmp4. (B) Addition of dorsomorphin significantly (p<0.001) enhanced spermatogonial cell proliferation in 3 week cultures. (C) Merged bright field and UV photomicrographs showing a colony containing approximately 65 DsRed-expressing spermatogonia. Data points not sharing a letter (A, B, C) are significantly different by Bonferroni–Dunn tests. Scale bar = 20 μm.
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