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Fig. 6
Co-localization of proliferating cells with GFAP, rhodopsin, and zpr-1.
Proliferating (PCNA-positive, red) cells in the inner nuclear layer (INL), but not in the outer nuclear layer (ONL: rod nucleus = RN, cone nucleus = CN), co-localized with GFAP (green) (arrows) at days 5 (A) and 15 (B). No rhodopsin is observed in the INL of untreated retina (C), whereas many PCNA-positive cells co-localized (arrows) with rhodopsin (green) in the ONL and INL during retinal regeneration after MNU exposure (D; day 15). No co-localization of zpr-1 stained double cones (green) and PCNA (red) was found (E, day 5). Cell nuclei are stained with DAPI (blue). Scale bar indicates 50 μm.