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Fig. S2
Sampling and quantifying methods for cell number and cell density. A: The 12 selected areas for measuring NCC and HR cell densities in the embryonic yolk sac surface. Unit area: 100 × 100 μm2. B: Regions of embryonic skin: head (I), yolk (II), and trunk (III). Scale bar = 100 μm. C: The middle part of 2nd gill arch for sampling and immunostaining. C-1, Areas of the gill filament (250 μm in total length) selected for cell density measurements. C-2, The area (filament and lamella within 250 µm in length) selected for determining total cell number.
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