Fig. 2
Development at low temperature rescues the s1pr2as10 cardiac phenotype.
(A) s1pr2as10 mutants raised at 28.5°C contained hearts with two atria (dark purple, amhc staining) and one ventricle (red, cmlc2 staining), and presented with tail blisters at 72 hpf. (B) s1pr2as10 mutant embryos raised at 22.5°C contained hearts with a single atrium and ventricle, and exhibited blood circulation at 72 hpf (arrow). (C) Percentages of s1pr2as10 mutant embryos raised at either 28.5 or 22.5°C containing two atria and one ventricle (2a1v) or one atrium and one ventricle (1a1v) at 72 hpf. (D) Percentages of s1pr2as10 mutant embryos raised at either 28.5 or 22.5°C displaying blood circulation at 72 hpf. The error bars indicate the standard error. Statistical significance was determined using Student’s t-test. *** indicates p<0.001.