IMAGE

Fig. 8

ID
ZDB-IMAGE-130907-33
Source
Figures for McCarthy et al., 2013
Image
Figure Caption

Fig. 8

Ethanol alters phosphorylation of AKT and pEIF4B in pdgfra mutants. Heads of 24 hpf pdgfra mutant (-/-) and pdgfra sibling (+/?) from untreated controls or embryos treated with 1.0% ethanol from 10-24 hpf were used. (A) Immunoblot was stripped and reprobed for total AKT, phospho-AKT (pAKT) and β-actin as a loading control. Both ethanol-treated pdgfra siblings and pdgfra mutants have elevated levels of phospho-AKT compared with untreated counterparts. (B) Ratio of pAKT to total AKT across three immunoblots. (C) Immunoblot was stripped and reprobed for total eIF4B (eIF4B), phospho-eIF4B (peIF4B) and β-actin as a loading control. pdgfra mutants have decreased levels of phospho-eIF4B compared with untreated mutants, and both untreated and ethanol-treated siblings samples. (D) Ratio of phospho-eIF4B to total eIF4B across three immunoblots.

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