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Fig. 7
Wortmannin or rapamycin induces pharyngeal skeleton hypoplasia in pdgfra mutants. (A-L) Embryos were treated at 10-24 hpf with (A-C) DMSO, (D-F) 1.0% ethanol, (G-I) 1.5 μM wortmannin or (J-L) 3 μM rapamycin. (M-O) Graphs depict the average area sizes, in thousands of μm2 in (M) the Meckel’s (mc), (N) palatoquadrate (pq) and (O) hyosymplectic (hs) cartilages. D, DMSO; E, ethanol; W, wortmannin, R, rapamycin [standard error bars are depicted at 1.5 s.e.m., significance is indicated by non-overlapping bars (Moses, 1987)]. Wild-type, light bar; pdgfra heterozygote, light-gray bar; mutant, dark-gray bar; ch, ceratohyal. Group numbers are the same as for Fig. 6.