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Fig. S2
pSmad1/5/9 expression in alk1-positive endothelial cells depends on alk1. pSmad1/5/9 expression (middle column) in endothelial cells (nuclei marked by fli1a:negfp transgene, left column) in wild-type, alk1-/- and alk1-/-;Tg(fli1a:alk1-myc) embryos at 36 hpf. In merge, EGFP-expressing endothelial cell nuclei are green, pSmad1/5/9 immunofluorescence is magenta. Yellow and blue arrows indicate endothelial cells in the CaDI and BCA, respectively. 2D confocal projections of 50 μm frontal sections, dorsal upwards. Scale bar: 50 μm. See Table S1 for fluorescence quantitation.
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