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Fig. 1
Dynamic Change in Global Myelination in the Larval Zebrafish Spinal Cord (A) Lateral views of the anterior (left) and posterior (right) spinal cord in Tg(mbp:EGFP-CAAX) animals at 4 days postfertilization (dpf) (top) and 8 dpf (bottom) indicate a significant increase in the number of myelin sheaths over this period. Scale bar, 20 μm. (B) Lateral views of the anterior (left) and posterior (right) spinal cord in Tg(mbp:EGFP) animals at 4 days postfertilization (dpf) (top) and 8 dpf (bottom) indicate a significant increase in the number of myelinating oligodendrocytes over this period. Scale bar, 20 μm. (C) Quantification of myelinating oligodendrocyte number in the anterior and posterior spinal cord at 4 and 8 dpf. Significance was tested using Student’s two-tailed unpaired t test. Error bars indicate SD. (D) Lateral views of a single axon labeled by cntn1b:mCherry and myelin sheaths labeled with Tg(mbp:EGFP-CAAX) at 4 dpf (top) and 8 dpf (bottom) show that the deposition of new myelin sheaths along single axons can occur over the course of several days. Arrowheads indicate putative nodes of Ranvier. Scale bar, 10 μm. (E) Quantification of the percentage axon length covered by myelin between 4 and 9 dpf. Each line represents a distinct axon. (F) Subset of axons shown in (E) where myelin sheath number increased over time. (G) Subset of axons shown in (E) where myelin sheath number does not change over time.
Reprinted from Developmental Cell, 25(6), Czopka, T., Ffrench-Constant, C., and Lyons, D.A., Individual Oligodendrocytes Have Only a Few Hours in which to Generate New Myelin Sheaths In Vivo, 599-609, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell