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Fig. S3
Representative images of hearts with EdU+ CMs. (A) Heart from control sibling embryo. (B) Heart from Tcf7l1a deficient embryo. (C) Heart from Tcf7l1b deficient embryo. (D) Heart from Tcf7l1 deficient embryo. MF20 (red) was used to label CMs. EdU+ cells (green; arrows in A and B) were easily discernible in the CMs and distinct from non-CMs when using the fluorescent stereomicroscope to focus through the hearts. The 30 min pulses of EdU labeled many additional cell types including blood, which can be seen in lumen of the hearts.
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Reprinted from Developmental Biology, 380(2), Sorrell, M.R., Dohn, T.E., D'Aniello, E., and Waxman, J.S., Tcf7l1 proteins cell autonomously restrict cardiomyocyte and promote endothelial specification in zebrafish, 199-210, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.