ZFIN Image: Mandal et al., 2013, Fig. 3

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Figure Caption

Fig. 3

Fluorescence in the Tg(β-actin:GDBD-RLBD);(UAS:EGFP) and Tg(β-actin:GDBD-RLBD);(UAS:mCherry) lines. A, E: Fluorescence in the Tg(β-actin:GDBD-RLBD);(UAS:EGFP) line is found in the spinal cord (arrow) by the 16s stage, although expression in the Tg(β-actin:GDBD-RLBD);(UAS:mCherry) is typically not visible by this stage. B, F: Expression at 24 hpf is visible in the spinal cord, notochord, and somites. Expression in the adjacent non-spinal cord tissues is more easily visible in the Tg(β-actin:GDBD-RLBD);(UAS:EGFP) embryos. C, D, G, H: Expression in maintained in the spinal cord, notochord, and somites at 30 and 48 hpf. Expression is seen in the ventral eye by 48 hpf (arrow in D). Arrowhead in D indicates the somites. Expression in the eye of the Tg(β-actin:GDBD-RLBD);(UAS:mCherry) embryo is from the α-cry:DsRed used in the transgenic construct. Arrow in H indicates autofluorescent pigment cells. All views are lateral with anterior up.

Acknowledgments

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