ZFIN Image: Wythe et al., 2013, Fig. 6

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Figure Caption

Fig. 6

ETS Factors Act Downstream of Vegf to Induce dll4 Expression (A) ERG mRNA (200 pg) injection partially rescued F2:GFP expression and sprouting (arrows) in vegfa morphants at 26 hpf. Representative images (left) and quantification (right, n = 10–12). (B) dll4 mRNA expression (normalized to kdr expression) was assessed by qRT-PCR in individual 26 hpf embryos injected with ERG mRNA and/or vegfa morpholino (n = 4). (C) Partial knockdown of erg and fli1a with a sub-phenotypic dose of morpholino inhibited the induction of F2:GFP expression in both the dorsal aorta (DA) and posterior cardinal vein (PCV) in Vegfa_121/165 mRNA-injected embryos. Arrows indicate collapsed PCV. Quantification of 10–13 embryos per group is shown. (D) Endogenous dll4 mRNA was assessed by qRT-PCR in individual embryos at 24 hpf (n = 5). All graphical data are mean ± SEM. See also Figure S6.

Acknowledgments

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Reprinted from Developmental Cell, 26(1), Wythe, J.D., Dang, L.T., Devine, W.P., Boudreau, E., Artap, S.T., He, D., Schachterle, W., Stainier, D.Y., Oettgen, P., Black, B.L., Bruneau, B.G., and Fish, J.E., ETS Factors Regulate Vegf-Dependent Arterial Specification, 45-58, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell