Fig. S5
ISH of muscle genes following Etv2 expression. Myog (A), myf6 (B), mylpfa (C), and tnnt3a (D) are all repressed in the trunk of embryos 4 h post–heat shock (HS+4 h). Normally these genes are strongly and specifically expressed in the musculature at this time point (control). Expression of myog and myf6 is almost completely abolished (A, B). Mylpfa and Tnnt3a are reduced but much less so (C, D). Quantification of the number of embryos demonstrating normal muscle expression over the number observed is in the bottom right corner of the corresponding high-magnification trunk image for each group. Four hours post–heat shock was chosen since it is the peak of heat shock–induced Etv2 expression.