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Fig. S5
Non heat-shocked control for the Tg(hsp70:dnigf1ra-GFP) fish experiment. BrdU incorporation was determined in non heat-shocked (Non-HS) Tg(hsp70:dnigf1ra-GFP) control fish (n = 4) (A and A′) and heat shocked (HS) Tg(hsp70:dnigf1ra-GFP) transgenic zebrafish (n = 6) (B and B′). Tg(hsp70:dnigf1ra-GFP) transgenic were heat shocked for 1 h at 38°C after amputation from 2–10 dpa. Non heat-shocked control fish were kept in the regular system. BrdU (green) and Mef2 (red) double positive cells indicate proliferating cardiomyocytes (A, A′, B, B′). A′ and B′ are the higher magnification images of the dashed boxes in A and B. The yellow box indicates the wound area; cardiomyocytes were counted in this region. BrdU (green) and Mef2 (red) staining were shown single channeled or merged color images. ia: injured area, v: ventricle. Scale Bar = 20 μm. (C) Quantification of BrdU positive cardiomyocytes (Mef2 positive) ± S.E. A significant decrease (*p<0.05) in cardiomyocyte proliferation was detected in Tg(hsp70:dnigf1ra-GFP) fish.