ZFIN Image: Huang et al., 2013, Fig. S2

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Figure Caption

Fig. S2

Inhibiting Igf signaling in zebrafish embryos results in mild defects in overall length. WT embryos were treated with DMSO as a control (A) (n = 20) or the Igf inhibitor NVP-AEW541 (B) (n = 20) from 48–72 hpf. Tg(hsp70:dnigf1ra-GFP) (D and E) and non-transgenic control (C) embryos were heat shocked twice at 48 and 72 hpf for 30 mins at 40°C. The embryos were observed at 76 hpf using a confocal microscope. Tg(hsp70:dnigf1ra-GFP) were identified by GFP expression. The length of the inhibitor treated embryos is about 3.2% shorter than untreated control embryos, and the Tg(hsp70:dnigf1ra-GFP) transgenic embryos are 5.7% % shorter than non-transgenic control embryos. Scale bar: (B) = 200 μm.

Acknowledgments

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