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Fig. 3
Igf signaling is required for cardiomyocyte proliferation during zebrafish heart development.
Wild type fish were treated with DMSO as a control (n = 5) (A and A′) and the Igf1r inhibitor NVP-AEW541 (n = 7) (B and B′) from 48–72 hpf. BrdU was added for the same time period. BrdU (green) and Mef2 (red) double positive cells indicate proliferating cardiomyocytes (A, A′, B, B′). A′ and B′ are images of the dashed boxes in A and B. (A′ and B′), BrdU (green) and Mef2 (red) staining were shown as black and white or merged color images. Scale bar: (B) = 50 μm, (B′) = 20 μm. v: ventricle. (C) Quantification of BrdU positive cardiomyocytes (Mef2 positive) ± S. E. A significant decrease (***p<0.0001) in cardiomyocyte proliferation was detected in embryos treated with NVP-AEW541.