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Fig. S6
Effect of Vatalanib treatment on vascular development of the trunk vasculature in zebrafish embryos and overall morphology. A–F2, tg(fli1:EGFP) zebrafish embryos were treated with Vatalanib in different concentrations for 48 hours, beginning at 48 hpf. Confocal images of trunk vasculature (A–F) and light images of overall morphology (A2–F2) were taken at 96 hpf. Embryos were incubated with control solution (0.05% DMSO in eggwater equal to the DMSO concentration in 10 μM Vatalanib treated embryos) (A, A2), 0,1 μM Vatalanib (B, B2), 1 μM Vatalanib (C, C2), 2 μM Vatalanib (D, D2), 5 μM Vatalanib (E, E2) and 10 μM Vatalanib (F, F2). ISV = intersomitic vessel, SIV = subintestinal vein plexus, PL = parachordal lymphangioblasts, DLAV = dorsal longitudinal anastomotic vessel. White scale bar: 100 μm; black scale bar: 500 μm.