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Fig. 6
PKD1 expression silencing disturbed formation of thoracic duct in 120 hpf tg(fli1:EGFP) zebrafish embryos.
A–A2, Bright-field image (A) and confocal microscopy (A2) of 120 hpf Co-Mo - injected (2 ng) zebrafish embryo showed physiological formation of thoracic duct (TD). The blue box marks the region shown in (A2). The red box marks the region shown in (B–E). DA: dorsal aorta, CV: cardinal vein, ISV: intersomitic vessel, TD: thoracic duct. C–D, Confocal microscopy of 120 hpf SB-Mo I5 - injected (500 pg) (C) and SB-Mo I6 - injected (500 pg) (D) embryos indicated a complete absence of TD (asterisks). E, Confocal microscopy of 120 hpf coinjected (500 pg SB-Mo I6 and 100 pg PKD1 mRNA) embryos showed a rescue effect on TD formation. F, Quantification of embryos with defects injected with Co-Mo (2 ng), SB-Mo I5 (500 pg), SB-Mo I6 (500 pg) or coinjection (SB-Mo I6 and mRNA). At least 30 embryos per group were analyzed. Blue bar indicates dorsal aorta (DA), red bar cardinal vein (CV) and arrows and dotted line the TD. Scale bars: 100 μm.