ZFIN Image: Sah et al., 2013, Fig. 4

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Figure Caption

Fig. 4

TRPM7 is highly expressed in murine SAN. (A) Confocal images of an isolated SAN freshly dissociated from adult αMHC-Cre-ROSA26^mTmG heart: differential interference contrast (Left) and fluorescence GFP image (Right). (Scale bar, 50 μm.) (B) PCR across exon 17 from genomic DNA isolated from dissected KO^αMHC-Cre and WT SAN. Tail DNA from Trpm7^fl/- (-) serves as a positive control for Trpm7 exon 17 deletion. Black arrow, full-length exon 17. Red arrow, deleted exon 17. (C) Representative TRPM7 current-voltage traces measured in WT SAN, WT AVM, and KO^αMHC-Cre SAN. (D) Mean TRPM7 current density in WT SAN (I_Trpm7, _WT _SAN = 32.0 ± 6.2 pA/pF, n = 6), WT AVM (I_Trpm7, _AVM = 8.3 ± 0.9 pA/pF, n = 5), and SAN (I_Trpm7, _KOαMHC-Cre _SAN = 0.4 ± 0.2 pA/pF, n = 4, P < 0.01).

Acknowledgments

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