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Fig. 4
Ablation of iridophores by Mtz treatment of fish injected with pnp4a:NTR.
(A) Iridophores in a wild-type larva (6.5 SSL) were marked by Venus fluorescence following injection of pnp4a:nlsVenus-V2a-NTR plasmid as the 1-cell stage, as shown in bright-field (A), fluorescence (A2) and merged (A3) views. (B–D) The same larva following Mtz treatment exhibited fewer, rounded iridophores that were progressively lost over several days. Inset in B shows reflecting-platelet containing extrusion bodies at the surface of the epidermis. Scale bar: in (A3) 60 μm for (A–D).
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