|
Fig. S1 (A) Expression of the miR-30 family as determined by in situ hybridisation at 8, 16 and 26 hpf. Embryos are orientated anterior to the left and dorsal up. Expression is ubiquitous with predominant expression in the cerebellum, retina and somites, as indicated (arrows). miR-30e shows additional expression in the linear heart tube (arrowhead) (B) Negative control in situ hybridisation using a sense miR-159 LNA probe shows no detectable expression at 8 hpf, 16 hpf and 24 hpf. (C) Negative control morpholino against miR-140 showed no detectable phenotype when injected at the same concentration as the miR-30 morpholino upto 3 dpf.