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Fig. 7
Observations made in vivo using the fundus lens are comparable to those made ex vivo. Images were captured in vivo at 10 days post-lesion (DPL), followed by sacrifice the same day to compare imaging results. In vivo images obtained with the fundus lens [A] were compared to the flatmounted retina dissected away from other ocular tissues [B]. Images A and B show ultraviolet (UV)-sensitive cones in the green fluorescent protein (GFP) channel. White box represent corresponding areas between in vivo and ex vivo; the distortion of the boxes seen in vivo is a result of flattening the curvature of the eye for best visualization during dissection. Arrows and chevrons indicate matching clusters of UV cones. Blood vessels were removed during dissection, so are not visible ex vivo. Background fluorescence is due to preservation of the dissected retina in fixative before mounting.