ZFIN Image: Albacker et al., 2013, Fig. 1

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Figure Caption

Fig. 1

An overexpression screen reveals SUV39H1 as a suppressor of rhabdomyosarcoma formation in zebrafish.

(A) Three linearized DNA constructs were injected into one-cell stage embryos, rag2-hKRAS^G12D, rag2-chromatin factor, and mylz2-GFP. At 2 dpf, embryos were scored for GFP mosaicism; only those that were GFP-high were kept for tumor evaluation. Tumor-free survival curves were then constructed for days 12–50 of life, looking for enhancers or suppressors of tumor formation compared to control injected zebrafish with rag2-hKRAS^G12D without a modifier gene. (B) SUV39H1 significantly suppressed RMS formation, compared to a control curve where the modifier gene was dsRed (SUV39H1 n = 73, dsRed n = 80, p = 0.0001).

Acknowledgments

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