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Fig. S2 Severe example of β-cell number reduction and disorganization in a hnf1bas430 mutant with mild exocrine hypoplasia. (A-D) Fluorescent confocal microscopy of Tg(ptf1a:eGFP)jh1 (green) 3 dpf wild-type (A,B) and 10 dpf hnf1bs430 mutant (C,D) pancreas stained for insulin to mark β-cells (red). 3D rendering showing an hnf1bas430 mutant at 10 dpf with a larger exocrine pancreas (C) than that of a wild type at 3 dpf (A). (B,D) Magnification (8×) of A and C showing that β-cells (red) remain both reduced and disorganized in hnf1bas430 mutants at 10 dpf.
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