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Fig. 6 Anti-inflammatory effect of genistein and sophoraisoflavone A.
A to C, zebrafish larvae are 4 dpf (days post-fertilization) with anterior to the left, scale bar = 10 μm. Migrating leukocytes were counted on one side in the tail in the region to the right of the dashed red arc and migration values were expressed as relative leukocyte migration (RLM) (C). A, negative control (DMSO 1%); B, genistein 25 μM; C, sophoraisoflavone A 25 μM; D, graph displaying the RLM in 4 dpf larvae (n = 10) after treatment with genistein and sophoraisoflavone A. RLM d0.5 was established as cutoff for anti-inflammatory activity. * p<0.05.