ZFIN Image: Teslaa et al., 2013, Fig. S5

Image

Figure Caption

Fig. S5 Transplanted cells contribute to neural crest migratory streams and craniofacial cartilage. (A, B) Eight micron confocal Z-stacks of host sox10:GFP+ embryos with alexa568+ transplanted cells. (A) Transplanted control morphant cells colocalize with sox10:GFP-positive cells in migratory streams in 5/7 embryos analyzed. (B) Transplanted zic2a morphant cells colocalize with sox10:GFP-positive cells in migratory streams in 3/5 embryos analyzed. (C–E) 4 dpf embryos stained for alcian blue and biotin. (C) Zic2a morphant embryos have typical bilateral defects in the pharyngeal cartilages. (D) Zic2a morphant embryos that receive transplanted control morphant cells frequently display more severe defects on one side (see arrows, 9/15, 1 exp.). (E) Biotin-positive control morphant cell incorporation in the ethmoid plate of a zic2a morphant host embryo (see arrow, 2/15, 1 exp.).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 380(1), Teslaa, J.J., Keller, A.N., Nyholm, M.K., and Grinblat, Y., Zebrafish Zic2a and Zic2b regulate neural crest and craniofacial development, 73-86, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.