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Fig. 6 Az treatment triggers mature lateral line supporting cells to re-enter the cell cycle. Images show BrdU labeling (red) or BrdU overlaid on DAPI (blue) labeling all nuclei. Dashed lines represent the extent of the neuromast. A: Fish treated with DMSO from 6 to 8 dpf, with BrdU present continuously in the media, have few BrdU-positive proliferating cells in their neuromasts, as most supporting cells have become quiescent. B: Fish treated with 2.5 μM Az with BrdU present continuously from 6–8 dpf, show increased numbers of BrdU-positive proliferating cells throughout the neuromast. C: Quantification of BrdU-positive cells in neuromasts from DMSO-treated or Az-treated fish from 6–8 dpf, separating them by location either at the periphery of the neuromast or internal. Az stimulates a more than doubling in the number of proliferating cells, both among the peripheral and internal supporting cell populations. Scale bar = 10 μm.