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Fig. 1 sulf1 is expressed in the developing notochord and trunk musculature in zebrafish embryos. (A–C) Lateral (A) or dorsal (B and C) views of zebrafish embryos fixed at 12 hpf and labeled with antisense probe against sulf1. Expression is strongest in the tailbud and notochord. (D and E) Lateral (D) or ventral (E) views of sulf1 expression in 18 hpf zebrafish embryos. Expression remains strong in the tailbud and notochord, but is also expressed in forebrain, otic vesicle, and somites. (F–H) Expression of sulf1 in 24 hpf zebrafish embryos. (F) Whole mount labeling showing expression in trunk musculature, along with midbrain–hindbrain boundary and optic stalks. (G) Cross-section at approximately the level of the line in (F) showing expression in medial trunk musculature and in the notochord and neural floorplate. (H) Higher magnification view of sulf1 expression in the tail. Scale bars: A, B, D, E, F: 250 μm; C: 25 μm; G: 50 7mu;m; H: 100 μm.
Reprinted from Developmental Biology, 378(2), Meyers, J., Planamento, J., Ebrom, P., Krulewitz, N., Wade, E., and Pownall, M.E., Sulf1 modulates BMP signaling and is required for somite morphogenesis and development of the horizontal myoseptum, 107-21, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.