ZFIN Image: Schmid et al., 2013, Fig. 6

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Figure Caption

Fig. 6 Severely degenerated myocytes of tardbp^-/-;tardbpl^-/- mutants. (A) Antibody staining of 2-dpf-old wild-type and tardbp^-/-;tardbpl^-/- mutant embryos with the myosin specific antibody ZE-BO-1F4 (green) and DAPI (blue). White arrowheads indicate degenerated myocytes. (B) Antibody staining of 1.5-dpf-old wild-type and tardbp^-/-;tardbpl^-/- mutant embryos with α-actinin (green), vinculin (red), and DAPI (blue). (A and B) Anterior to the left, lateral view. (Scale bars, 20 μm.) (C) EM pictures of skeletal muscle of a tardbp^-/-;tardbpl^-/- mutant embryo shows a highly disorganized pattern of thinner myofibrils (f) with disorganized network of sarcoplasmic reticulum (open arrowheads). Large part of the sarcoplasmic reticulum is misaligned and dilated (filled arrowheads). Individual myofibrils are not well separated from another and mitochondria can now be found within the muscle fibers at (black arrow). (2 dpf) (Scale bars, 500 nm.)

Acknowledgments

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