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Fig. 5 EGPF expression driven by HAR96 in stable and transient transgenic zebrafish lines at 24 and 48 hpf. (A) Diagram of the transgene structure based on the Tol2 system. Lower panel, fluorescent (B, C, F, and G) and bright field (D, E, H, and I) photomicrographs of selected whole mount transient or stable transgenic zebrafish embryos expressing the reporter protein EGFP under control of the human HAR96 sequence at 24 hpf (B, D, F, and H) and 48 hpf (C, E, G, and I). Coronal cryostat sections showing a detail of the EGFP expression pattern at three levels (white vertical bars in G) of the 48 hpf developing zebrafish (J, K, and L). Below, cells expressing EGFP (M and P) the early neuronal marker HuC/D (N and Q) and the resulting overlay (O and R) in the 48 hpf developing zebrafish (sections levels are approximately indicated in G). TeO, tectum opticum; T, midbrain tegmentum; DT, dorsal tegmentum; Po, preoptic region; MO, medulla oblongata; OC, otic capsule; SC, spinal cord; Hi, hypothalamus; Hy, hypophysis.