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Fig. 7

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ZDB-IMAGE-130624-21
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Figures for Wang et al., 2013
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Fig. 7 Notch signaling was not activated in the AVC of scote382 mutant hearts. (AF) Tg(Tp1bglob:hmgb1-mCherry)jh11, called Tg(Tp1:mCherry), transgenic zebrafish (Parsons et al., 2009) were bred with scote382/te382; Tg(kdrl:eGFP) transgenic zebrafish to generate scote382/+; Tg(Tp1:mCherry)/+; Tg(kdrl:eGFP)/+ zebrafish. Images were taken with agarose-embedded embryos using Zeiss 700 upright confocal microscope. Endocardial cells labeled by Tg(kdrl:eGFP) were used to project the heart shapes. Note that Tg(Tp1:mCherry) were gradually restricted in the AVC of wild-type hearts at 48hpf (A), 60hpf (C) to 72hpf (E), but were ectopically expressed in the ventricle of scote382 mutant hearts at 48hpf (B), 60hpf (D) and 72hpf (F). a, atrium; v, ventricle. Red arrowheads point to the AVC.

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