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Fig. 6 Germline transmission of 6-week cultured FGSCs.
(A) Photomicrograph showing the incorporation of DsRed-positive cultured FGSCs (arrow) into the gonad of a recipient larva two weeks after transplantation. (B) Results of genomic PCR showing the presence of ziwi-neo sequences that were inherited by all of the F1 individuals (lanes 1 to 16) produced by a germline chimeric father. Negative control: genomic DNA template from a wild-type larva (lane 17); positive control: pZiwi-neo plasmid DNA template (lane 18). (C) Dissection of a fertile adult male recipient fish showing that the transplanted DsRed-positive FGSCs have proliferated and directed the formation of a testis (arrow) on one side of the body. (C1) Inset shows the gonad under UV light revealing the presence of DsRed-positive cells. (D) Transverse section of testis from a fertile recipient fish showing active spermatogenesis. S: spermatozoa. Scale bar = 50 μm for A, C and 20 μm for D.