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Figure Caption
Fig. 3 Effect of OFC feeder lines on FGSC cultures.
Photomicrograph showing G418-selected FGSCs that were initiated from Tg(ziwi:neo);Tg(ziwi:DsRed) zebrafish and grown on OFC feeders showing a (A) four-cell and (B) eight-cell colonies, black arrows; (C) DsRed expression in the eight-cell colony; (D) merged images of B and C. (E) OFC3 significantly (p = 0.002) promoted FGSC colony formation. (F) Both OFC1 and OFC3 significantly (p = 0.0002) increased the number of FGSCs in a 3-week culture. Data points not sharing a letter (A, B, C) are significantly different by Bonferroni–Dunn tests. Scale bar = 20 μm.
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