ZFIN Image: Kimura et al., 2013, Fig. 2

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Figure Caption

Fig. 2 Activation of Hindbrain V2a Neurons Elicits Swimming Compound transgenic fish of Tg[chx10:Gal4] and Tg[UAS:ChR] at 3 dpf were used in the experiments. (A and B) A flash of blue light (0.4 mW/mm², 100 ms) was applied to the boxed area (left panel in A). This evoked swim-like behavior (right panels in A). (B) Frequencies of eliciting swim-like behavior. “WT” represents wild-type larvae, whereas “ChR” represents larvae that expressed ChR in V2a neurons. n = 35, 7 animals for both cases. (C and D) Region-specific photo stimulations were applied. The hindbrain was divided into three regions (b, rostral; c, middle; d, caudal). Photo stimulations were applied to each region. In addition, photo stimulations were applied to the midbrain (a) and spinal cord (e). (D) Frequencies of eliciting swim-like behaviors with different stimulation sites and different stimulation strengths. The duration of the stimulation was 100 ms.

Acknowledgments

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