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Fig. S2 Suppression of Wnt activity by rspo3 knockdown. (A-C) Expression of GFP protein in the Wnt reporter line tg(7xTCFXla. Siam:GFP)ia4 in conMO (A), rspo3-MO (B) and lef1-MO (C) morphants. Knockdown of rspo3 reduces GFP expression (B). Knockdown of lef1 inhibits GFP expression (C). (D,E) Quantification of GFP signal intensity using the photon-counting mode on a Leica hybrid detector (D), and photon counts per square micron for the GFP-positive portion of the PLLp (E). *P<0.05, **P<0.01.
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