Fig. S3 Gonads from sex-reverted Tg(ziwi:CFP-NTR) females contain testis-like tissue. (A) Ovaries of control DMSO-treated females have many large, yolky oocytes. (B) Gonads of Mtz-treated sex-reversed fish contain mostly adipose tissue with patches of opaque testis-like tissue, most similar to the testes of a control male (E). (C, D, F, G) Fluorescent micrographs of the gonad of an Mtz-treated 1° female and testes of control male with CFP pseudocolored in green. (C) Gonads from Mtz-treated females contain patches of CFP-positive cells that resemble control testis tissue (F). (D, G) Magnified views of the boxed regions in C and F, respectively.
Reprinted from Developmental Biology, 376(1), Dranow, D.B., Tucker, R.P., and Draper, B.W., Germ cells are required to maintain a stable sexual phenotype in adult zebrafish, 43-50, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.