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Fig. 6 Zebrafish pnpla6 acts cell-autonomously in motor neurons. (A-H) RLDx-labeled cells from embryos injected with control MO or pnpla6 MO were transplanted into wild-type embryos. (A) Homotopic transplantation of control or pnpla6 morphant cells. The cell mass injected is encircled. (B) Number of sides with at least one of the specified motor axon defects. (C,F) Immunostaining of embryos with antibodies to Znp-1 (green). (D,G) View of RLDx-labeled (red) donor cells in recipients. (E,H) In merged images, motor neurons from pnpla6 morphants (H) exhibit aberrant truncation and branching compared with control (E). Scale bars: 200 μm (A,B); 50 μm (C-H).