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Figure Caption
Fig. 2 Rescue of pnpla6 MO phenotype with human PNPLA6 mRNA. (A) Clustal alignment of partial Pnpla6 protein sequence in human, mouse and zebrafish. The NEST/patatin domain (blue line) is highly conserved. Three key conserved amino acids for the catalytic activation are shown in boxes. (B-G) Rescue of curly-tail phenotype. The abnormal phenotype caused by pnpla6 knockdown can be rescued by co-injection of human PNPLA6 mRNA, but not mutant human mRNA (D960A, S966A or D1086A mRNAs). (H) Quantification of embryos with curly tail; *P<0.001 compared with control MO group. Scale bar: 200 μm.
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Full text @ Dis. Model. Mech.