|
Fig. 5 Ptk7 overexpression inhibits exogenous non-canonical Wnt/PCP activity. (A) Flat-mounts of 10- to 12-somite stage wild-type (WT) embryos and embryos injected with ptk7 (200 pg), wnt5b (50 pg) or ptk7 (200pg) + wnt5b (50 pg) mRNA stained for krox20 (hindbrain) and myoD (somite) gene expression. (B) Quantification of the anterior-posterior extent of the myoD expression domain in WT, ptk7 (200 pg), wnt5b (50 pg), and ptk7 (200 pg) + wnt5b (50 pg) mRNA-injected embryos (***P<0.001). n.s., not significant. (C) Ventral views of embryos at 2 days post-fertilisation (dpf). Cyclopia phenotypes were observed as class I (no cyclopia), class II (partial cyclopia) and class III (full cyclopia). (D) Quantification of cyclopia phenotypes in WT, ptk7 (200 pg), wnt11 (50 pg), and ptk7 (200 pg) + wnt11 (50 pg) mRNA-injected embryos at 2 dpf.