Fig. S6

Fig. S6 MZptk7^hsc9 mutants display tailbud patterning defects. (A,B) Whole-mount in situ hybridization of mespb expression, which delineates the anterior extent of pre-somitic mesoderm in wild-type (A) and MZptk7^hsc9 mutant (B) embryos. Mespb expression is shifted anterior relative to total body axis length in MZptk7^hsc9. (C) Quantification of the ratio of the distance between the most posterior part of the embryonic tail (bracket in A) and the most posterior extent of the mespb expression domain to the total body axis length (dashed line in A) from head to tail in wild-type (WT) and MZptk7^hsc9 at the 10- to 12-somite stage. Student’s t-test, **P=0.0016. Error bars represent s.d.