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Fig. S4 Plasma membrane-tethered extracellular domain is required for Ptk7 overexpression activity. (A) Class of phenotypes induced by ptk7 overexpression: wild-type/class 1; class 2, axial extension defects as well as dorsal curvatures of the posterior tail; class 3, mild to severe dorsalisation. (B) Distribution of phenotypes in embryos injected with ptk7 (400 pg), ptk7ΔICD (300 pg), ptk7 egfrTM (400 pg), ptk7ΔECD (300 pg) and ptk7 ECD (300 pg) mRNA.
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