ZFIN Image: Wolf et al., 2013, Fig. S17

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Figure Caption

Fig. S17 Heat shock induces overexpression of the transgenes. (A-H) After an overnight heat shock, 2-dpf wild-type embryos (A,C,E,G) and embryos containing Tg(hsp70:otpa-2A-EGFP)hd7 (B), Tg(hsp70:sim1a-2A-lyntdTomato)hd8 (D), Tg(hsp70:fezf2-2AEGFP) hd6 (F) and Tg(hsp70:foxb1.2-2A-EGFP)hd9 (H) were subjected to WISH to detect the overexpression of otpa, sim1a, fezf2 and foxb1.2. Transgenic embryos expressing otpa (B; n=24/24), sim1a (D; n=29/29), fezf2 (F; n=37/37) and foxb1.2 (H; n=34/34) show overexpression of the specific transgenes. Lateral views are shown. hs, heat shock; OE, overexpression. Scale bar: 100 μm.

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