|
Fig. S14 Axon tracts are present in the posterior hypothalamic area. (A-C′′) Immunohistochemistry (IHC) of 1-dpf (A-A′′), 2-dpf (B-B′′) and 3-dpf (C-C′′) wild-type embryos using antibodies detecting acetylated tubulin (A,B,C) and Otpa (A′′ ′,B′′ ′,C′′ ′). Maximum projections of up to 25 μm stacks in the lateral view are shown. The white arrows point to the Otpa-positive MA. (A-A′′) At 1 dpf the posterior Otpa-positive domain is located near dense axon tracts. (B-C′′) Also at 2 and 3 dpf, axon tracts are found in the Otpa-positive MA. ac, anterior commissure (ac); MA, mammillary area. Scale bar: 60 μm.