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Fig. S6 The hypothalamic fgf8 expression domain is expanded in embryos lacking fezf2 activity. (A-B′′) Two-color FISH to detect the relative expression of fgf8 and nkx2.1a in 1-dpf wild-type embryos (A-A′′) and fezf2 morphants (B-B′′). fgf8 is co-expressed with nkx2.1a (arrows) and the hypothalamic expression domain of fgf8 is expanded in embryos lacking fezf2 activity (B-B′′). Maximum projections of 10 μm confocal stacks in the lateral view are shown. LOF, loss-of-function. Scale bar: 60 μm.
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