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Fig. S3

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ZDB-IMAGE-130429-17
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Wolf et al., 2013 - Specification of posterior hypothalamic neurons requires coordinated activities of Fezf2, Otp, Sim1a and Foxb1.2
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Fig. S3 Adenohypophysis markers are not expressed in the hypothalamus at 1 dpf. Three-color FISH of 1-dpf wild-type embryos to detect the expression of pit1, crabp1a, prl, nkx2.1a, fezf2 and emx2. (A-B-′′′) pit1 (arrows) is not co-expressed with nkx2.1a (A-A′′), fezf2 or emx2 (B-B-′′′). (C-C-′′′) crabp1a (arrowhead) is partially co-expressed with fezf2 but not with emx2. Lateral views of maximum projections of 10 μm confocal stacks are shown. Scale bar: 60 μm.

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